LOCUS       V003731                 7062 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003731
VERSION     V003731
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Okamoto S, Niki H.
  TITLE     NBRP cloning vector collection sequence project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Okamoto S, Niki H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2017) Contact:Sho Okamoto National Institute of
            Genetics, Microbial Genetics Laboratory; 1111 Yata, Mishima,
            Shizuoka 411-8540, Japan URL
            :https://www.nig.ac.jp/labs/MicroGen/index.html
REFERENCE   3  (bases 1 to 7062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-APR-2017) Contact:Sho Okamoto National Institute of Genetics,
            Microbial Genetics Laboratory; 1111 Yata, Mishima, Shizuoka
            411-8540, Japan URL :https://www.nig.ac.jp/labs/MicroGen/index.html"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1260..1907
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2663..2677)
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             3773..3943
                     /label="ProtA"
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     misc_feature    4467..4522
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        4996..5100
                     /label="AmpR promoter"
     CDS             5101..5958
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gatcaatttc ttttaagtaa tctaaatccc cattttttaa tttcttttta gcctctttaa
       61 ataatcctga ataaactaat acctgtttac ctttaagtga tttataaaat gcatcaaaga
      121 ctttttgatt tatttattaa ataatcacta tctttaccag aatacttagc catttcatat
      181 aattctttat tattattttg tcttattttt tgaacttgaa cttgtgttat ttctgaaatg
      241 cccgttacat cacgccataa atctaaccat tcttgttggc taatataata tcttttatct
      301 gtgaaatacg atttatttac tgcaattaac acatgaaaat gaggattata atcatctctt
      361 tttttattat atgtaatctc taacttacga acatatccct ttataacact acctactttt
      421 tttctcttta taagttttct aaaagaatta ttataacgtt ttatttcatt ttctaattca
      481 tcactcatta cattaggtgt agtcaaagtt aaaaagataa actccttttt ctcttgctgc
      541 ttaatatatt gcatcatcaa agataaaccc aatgcatctt ttctagcttt tctccaagca
      601 cagacaggac aaaatcgatt tttacaagaa ttagctttat ataatttctg tttttctaaa
      661 gttttatcag ctacaaaaga cagaaatgta ttgcaatctt caactaaatc catttgattc
      721 tctccaatat gacgtttaat aaatttctga aatacttgat ttctttgttt tttctcagta
      781 tacttttcca tgttataaca cataaaaaca acttagtttt cacaaactat gacaataaaa
      841 aaagttgctt tttccccttt ctatgtatgt tttttactag tcatttaaaa cgatacatta
      901 ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat aacttaaggg
      961 taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
     1021 cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
     1081 tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
     1141 aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
     1201 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     1261 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     1321 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     1381 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     1441 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     1501 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     1561 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     1621 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     1681 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     1741 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     1801 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     1861 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     1921 tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
     1981 gaaggttttt atattacagc tccagatcca tatccttctt tttctgaacc gacttctcct
     2041 ttttcgcttc tttattccaa ttgctttatt gacgttgagc ctcggaaccc ttaacaatcc
     2101 caaaacttgt cgaatggtcg gcttaatagc tcacgctatg ccgacattcg tctgcaagtt
     2161 tagttaaggg ttcttctcaa cgcacaataa attttctcgg cataaatgcg tggtctaatt
     2221 tttattttta ataaccttga tagcaaaaaa tgccattcca atacaaaacc acatacctat
     2281 aatcgataac cacataacag tcataaaacc actccttttt aacaaacttt atcacaagaa
     2341 atatttaaat tttaaatgcc tttattttga attttaaggg gcattttaaa gatttagggg
     2401 taaatcatat agttttatgc ctaaaaacct acagaagctt ttaaaaagca aatatgagcc
     2461 aaataaatat attctaattc tacaaacaaa aatttgagca aattcagtgt cgatttttta
     2521 agacactgcc cagttacatg caaattaaaa ttttcatgat tttttatagt tcctaacagg
     2581 gttaaaattt gtataacgaa agtataatgt ttatataacg ttagtataat aaagcatttt
     2641 aacattatac ttttgataat cgtttatcgt cgtcatcaca ataactttta aaatactcgt
     2701 gcataattca cgctgacctc ccaataacta catggtgtta tcgggaggtc agcacttgtg
     2761 ttttctattt tttaataaaa cctcagcaca taatgaacaa ctttctattt tctatatcac
     2821 ttaaaaccat ttccgaaatt aaacctcagc acattcaaag ccccacttta ttcttaaaaa
     2881 tattttttaa ctcatatgta ttaaaccgct ttcattataa aaaatatctc tatattttat
     2941 ctgtttttat taatcgaaat agcgtgattt tgcggtttta agccttttac ttcctgaata
     3001 aatctttcag caaaatattt attttataag ttgtaaaact tacatttaaa tttaattata
     3061 aatatagatt ttagtattgc aatacataat tcgttatatt atgatgactt tacaaataca
     3121 tacagggggt attaatttga aaaagaaaaa catttattca attcgtaaac taggtgtagg
     3181 tattgcatct gtaactttag gtacattact tatatctggt ggcgtaacac ctgctgcaaa
     3241 tgctgcgcaa cacgatgaag ctcaacaaaa tgctttttat caagtcttaa atatgcctaa
     3301 cttaaatgct gatcaacgca atggttttat ccaaagcctt aaagatgatc caagccaaag
     3361 tgctaacgtt ttaggtgaag ctcaaaaact taatgactct caagctccaa aagctgatgc
     3421 gcaacaaaat aacttcaaca aagatcaaca aagcgccttc tatgaaatct tgaacatgcc
     3481 taacttaaac gaagcgcaac gtaacggctt cattcaaagt cttaaagacg acccaagcca
     3541 aagcactaac gttttaggtg aagctaaaaa attaaacgaa tctcaagcac cgaaagctga
     3601 taacaatttc aacaaagaac aacaaaatgc tttctatgaa atcttgaata tgcctaactt
     3661 aaacgaagaa caacgcaatg gtttcatcca aagcttaaaa gatgacccaa gccaaagtgc
     3721 taacctattg tcagaagcta aaaagttaaa tgaatctcaa gcaccgaaag cggataacaa
     3781 attcaacaaa gaacaacaaa atgctttcta tgaaatctta catttaccta acttaaacga
     3841 agaacaacgc aatggtttca tccaaagcct aaaagatgac ccaagccaaa gcgctaacct
     3901 tttagcagaa gctaaaaagc taaatgatgc tcaagcacca aaagctgaca acaaattcaa
     3961 caaagaacaa caaaatgctt tctatgaaat tttacattta cctaacttaa ctgaagaaca
     4021 acgtaacggc ttcatccaaa gccttaaaga cgatccttca gtgagcaaag aaattttagc
     4081 agaagctaaa aagctaaacg atgctcaagc accaaaagag gaagacaata acaagcctgg
     4141 caaagaagac aataacaagc ctggcaaaga agacaacaac aagcctggta aagaagacaa
     4201 caacaagcct ggcaaagaag acggcaacaa gcctggtaaa gaagacaaca aaaaacctgg
     4261 taaagaagat ggcaacaagc ctggtaaaga agacaacaaa aaaacctggt aaagaagacg
     4321 gcaacaagcc tggcaaagaa gatggcaaca aacctggtaa agaagatggt aacggagtac
     4381 atgtcgttaa acctggtgat acagtaaatg acattgcaaa agcaaacggc actactgctg
     4441 acaaaattgc aagcctggtg gaccaggaat tcgagctcgg tacccgggga tcctctagag
     4501 tcgacctgca ggcatgcaag ctagcttaag taagtaagta agccgccagt tccgctggcg
     4561 gcattttttt tgatatcatc gatacaatac acaacgatag atatcatttt atccaaacca
     4621 attttaactt atatacgttg attaacacat tcttatttga aatgataaga atcatctaaa
     4681 tgcacgagca acatcttttg ttgctcagtg cacatatggt gcactctcag tacaatctgc
     4741 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4801 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4861 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6901 gagcctgtta gcacttatat tttgttattg ttcttcctcg atttcgtcta tcattttgtg
     6961 attaatttct cttttttctt gttctgttaa gtcataaagt tcactagcta aatactcttt
     7021 ttgtttccaa atataaaaaa tttgatagat atattacggt tg
//
